期刊
JOURNAL OF NEURAL TRANSMISSION
卷 115, 期 11, 页码 1545-1556出版社
SPRINGER WIEN
DOI: 10.1007/s00702-008-0126-4
关键词
Advanced glycation endproducts; AGEs; Viability; Caspases 3 and 7 activities; Reactive oxygen species
资金
- Interdisciplinary Centre of Clinical Research (IZKF) at the Faculty of Medicine of the University of Leipzig [01KS9504, N1]
- Alzheimer Forschungs Initiative e.V. [01805]
- Deutsche Forschungsgemeinschaft (DFG) [MU 1011/14-1]
- Studienstiftung des deutschen Volkes
Advanced glycation endproducts (AGEs) arise from the reaction of sugars with side chains and the N-terminus of proteins and are thought to be involved in the pathogenesis of several diseases by inducing oxidative stress, inflammation and cell death presumably mediated through activation of the receptor of AGE (RAGE). To address the question whether the cell damaging effect of AGE depends on the degree of its protein glycation, differential modified AGEs derived from incubating human serum albumin with increasing concentrations of methyl glyoxal were tested on cell viability, reactive oxygen species (ROS) formation, intracellular ATP levels, and activation of caspases 3/7 in two human glial cell lines, which were used as a model for human glia cells. All AGEs tested, regardless of their degree of modification, were found to induce ROS formation in both microglial (CHME-5) and astroglial cells (U373 MG), while only highly modified AGEs were able to decrease the cell viability and to induce apoptosis. This indicates that apoptotic events may be involved in the change of physiological parameters.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据