A composite SWNT-collagen matrix: characterization and preliminary assessment as a conductive peripheral nerve regeneration matrix

Unique m their structure and function single-walled carbon nanotubes (SWNTs) have received significant attention due to their potential to create unique conductive materials For neural applications, these conductive materials hold promise as they may enhance regenerative processes However, like other nano-scaled biomaterials it is important to have a comprehensive understanding how these materials interact with cell systems and how the biological system responds to their presence These investigations aim to further our understanding of SWNT-cell interactions by assessing the effect SWNT/collagen hydrogels have on PC12 neuronal-like cells seeded within and (independently) on top of the composite material Two types of collagen hydrogels were prepared (1) SWNTs dispersed directly within the collagen (SWNT/COL) and (2) albumin-coated SWNTs prepared using the surfactant 'sodium cholate' to improve dispersion (AL-SWNT/COL) and collagen alone serving as a control (COL) SWNT dispersion was significantly improved when using surfactant-assisted dispersion The enhanced dispersion resulted in a stiffer, more conductive material with an increased collagen fiber diameter Short-term cell interactions with PC 12 cells and SWNT composites have shown a stimulatory effect on cell proliferation relative to plain collagen controls In parallel to these results, p53 gene displayed normal expression levels, which indicates the absence of nanoparticle-induced DNA damage In summary, these mechanically tunable SWNT collagen scaffolds show the potential for enhanced electrical activity and have shown positive in vitro biocompatibility results offering further evidence that SWNT-based materials have an important role in promoting neuronal regeneration