期刊
JOURNAL OF NATURAL PRODUCTS
卷 75, 期 1, 页码 67-71出版社
AMER CHEMICAL SOC
DOI: 10.1021/np200666t
关键词
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资金
- National Institute of Food and Drug Evaluation for Studies on the Standardization of Herbal Medicines
- Korean government (MEST) [20090083533]
Phytochemical investigation of Leonurus japonicus has led to the isolation of a labdane diterpene derivative, 15,16-epoxy-3 alpha-hydroxylabda-8,13(16),14-trien-7-one (1), which was tested for its in vitro anti-inflammatory effects. The results demonstrated that 1 exhibits an inhibitory effect on LPS-stimulated RAW 264.7 macrophages. The anti-inflammatory action shown by 1 suppressed LPS-induced NE-kappa B activation, resulting in the down-regulation of iNOS and COX-2 protein expression, attributable to the inhibitory action of LPS-induced NO and PGE(2) production. Compound 1 inhibited LPS-induced phosphorylation and the degradation of inhibitory kappa B (I kappa B alpha) and decreased the nuclear translocation of p50 and p65. In addition, 1 exhibited an inhibitory effect on LPS-induced NE-kappa B-DNA and AP-1-DNA binding activity, using an electrophoretic mobility shift assay with NE-kappa B- and AP-1-specific P-32-labeled probes. The LPS-induced mitogen-activated protein kinases (p-JNK, p-p38, and p-ERK) and p-Akt were inhibited after 30 and 10 min of LPS stimulation, respectively. In addition, TNF-alpha production was suppressed by 1.
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