期刊
JOURNAL OF NATURAL PRODUCTS
卷 75, 期 9, 页码 1652-1655出版社
AMER CHEMICAL SOC
DOI: 10.1021/np300444e
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资金
- China Scholarship Council (CSC)
- Deutsche Forschungsgemeinschaft (DFG)
- Bundesministerium fur Bildung und Forschung (BMBF)
- BMBF (MiPro)
The 18 kb silent luminmycin biosynthetic pathway from Photorhabdus luminescens was cloned into a vector by using the newly established linear-linear homologous recombination and successfully expressed in Escherichia coli. Luminmycins A-C (1-3) were isolated from the heterologous host, and their structures were elucidated using 2D NMR spectroscopy and HRESIMS. Luminmycin A is a deoxy derivative of the previously reported glidobactin A, while luminmycins B and C most likely represent its acyclic biosynthetic intermediates. Compound 1 showed cytotoxicity against the human colon carcinoma HCT-116 cell line with an IC50 value of 91.8 nM, while acyclic 2 was inactive at concentrations as high as 100 mu g/mL.
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