期刊
JOURNAL OF NATURAL PRODUCTS
卷 71, 期 8, 页码 1489-1508出版社
AMER CHEMICAL SOC
DOI: 10.1021/np800144q
关键词
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An assessment of the technology and method development in countercurrent chromatography (CCC) and centrifugal partition chromatography (CPC), collectively referred to as countercurrent separation (CS), is provided. More than six decades of CS theory and applications are critically reviewed and developed into a practical guide to CS for natural products research. The necessary theoretical foundation is given for better use of CS in the separation of biological molecules of any size, small to large, and from any matrix, simple to complex. The three operational fundamentals of CS-instrumentation, biphasic solvent systems, and theory-are covered in I prismatic fashion. The goal of this review is to provide the necessary background and references for an up-to-date perspective of CS and to point Out its Potential for the natural products Scientist for applications in natural products chemistry, metabolome, and proteome research involving organisms from terrestrial and marine sources.
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