Antifungal metabolites from the plant endophytic fungus Pestalotiopsis foedan

Pestafolide A (1), a new reduced spiro azaphilone derivative, and pestaphthalides A (2) and B (3), two new isobenzofuranones, have been isolated form solid cultures of an isolate of Pestalotiopsis foedan. The structures of these compounds were determined mainly by analysis of their NMR spectroscopic data. The relative configuration of 1-3 was assigned by analysis of H-1 NMR J-values and NOESY data, and the absolute configuration was determined by application of the CD excitation chirality and modified Mosher method. Compounds 1-3 showed modest antifungal activity.