期刊
JOURNAL OF NATURAL MEDICINES
卷 66, 期 1, 页码 177-184出版社
SPRINGER TOKYO
DOI: 10.1007/s11418-011-0573-3
关键词
HMC05; Human aortic smooth muscle cells; Tumor necrosis factor-alpha; MAPK signal pathway; HASMC migration and proliferation
资金
- Ministry of Health & Welfare, Republic of Korea [B080031]
- Dongguk University
- Korea Health Promotion Institute [B080031] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
HMC05 is a formulation derived from eight medicinal herbs, and prevents neointima formation by inhibition of the mitogen-activated protein kinase (MAPK) pathway with induction of heat shock protein 27 expression. In this study, we investigated the influence of HMC05 regulation on the MAPK/extracellular signal-regulated kinase (ERK) 1/2 signaling cascade in the inhibition of the migration and proliferation of human aortic smooth muscle cells (HASMCs). The inhibitory effects of HMC05 (25, 50, and 100 mu g/ml) on tumor necrosis factor-alpha (TNF-alpha; 0 or 100 ng/ml)-induced HASMC migration and proliferation were investigated by wound migration and proliferation assays, Western blotting and reverse transcription-polymerase chain reaction. HMC05 completely inhibited TNF-alpha-induced HASMC migration and proliferation. HMC05 prevented TNF-alpha receptor 1-mediated phosphorylation of signal transduction molecules involved in MAPK signaling cascades such as MEK1/2, ERK1/2, Elk-1 transcription factor and p90 kDa ribosomal S6 kinase. The expression of matrix metalloproteinase, a modulator of vascular smooth muscle cell proliferation and migration, was inhibited by HMCO5 treatment, as was TNF-alpha-induced mRNA expression of intracellular adhesion molecule 1 and vascular cell adhesion molecule 1. HMC05 disruption of the MEK/ERK/Elk-1 and p90RSK pathways prevents HASMC migration and proliferation.
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