Multiplexing Enhancement for the Detection of Multiple Pathogen DNA

Various methods for the detection of pathogens have been researched and developed. However, most of detection methods are cost-ineffective and laborious. To minimize costs and labor, multi-detection of pathogen have been widely used. In this p a p e we propose a novel multi-detection method, which can enhance multi-detection capability using in flu nano fluorescent dye labeled DNA nanostructures that is named DNA nanobarcodes. By using three fluorescence colors, multi-detection capability is significantly increased because of the increased combination of three colors. Moreover, our approach uses a relatively simple DNA nanostructure to precisely control the fluorescence intensity ratio. Therefore, high multi-detection ability is achieved without constructing a complicated DNA nanostructure probe. Our novel detection method can overcome the obstacles of conventional methods and enhance multi-detection capability effectively By using our new system, we were able to successfully detect nine different DNA pathogens simultaneously. Our system can easily increase the multiplexibility by using more fluosrescent colors on DNA nanobarcode.