期刊
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
卷 10, 期 4, 页码 2314-2324出版社
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/jnn.2010.1917
关键词
Mesoporous Silica Nanoparticles; Functionalization; Aminofluorescein; Uptake; Endocytosis; Cytotoxicity; HeLa Cells
类别
资金
- French National Research Agency (ANR) [ANR-05-BLAN-0164]
- Agence Nationale de la Recherche (ANR) [ANR-05-BLAN-0164] Funding Source: Agence Nationale de la Recherche (ANR)
Mesoporous silica nanoparticles (MSN) were functionalised by aminofluorescein (AMF) with diethylenetriaminepentaacetic acid spacer molecules which provide free carboxylic groups for binding cell-specific ligands such as folate. AMF allowed the exploration of cellular uptake by HeLa cells using confocal microscopy and flow cytometry. The functionalized nanoparticles (MSN-AMF) penetrated efficiently into HeLa cell cytoplasm through a clathrin dependent endocytosis mechanism. The number of endocytosed MSN-AMF was enhanced when using folate as a targeting molecule. Uptake kinetics revealed that most of MSN-AMF were internalized within 4 h of incubation. Moreover, we found that MSN-AMF were capable of escaping the acidic endolysosomal vesicles of HeLa cells. Cytotoxicity studies suggested that these nanoparticles are non-toxic to HeLa cells up to a dose level of 50 mu g/ml.
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