期刊
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
卷 10, 期 2, 页码 941-947出版社
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/jnn.2010.1892
关键词
Glucose Sensor; Glucose Oxidase; Direct Electron Transfer; Carbon Nanotube Mat; Amperometry; Mediator-Less
类别
资金
- NSF Center for Scalable and Integrated Nano Manufacturing (SINAM) [DMI-NSEC-0327077]
- NSF IGERT: Material Creation Training Program (MCTP) [DGE-0654431]
- California NanoSystems Institute
- Directorate For Engineering [0751621] Funding Source: National Science Foundation
- Div Of Civil, Mechanical, & Manufact Inn [0751621] Funding Source: National Science Foundation
In this paper, the direct electron transfer of glucose oxidase (GOx) on carbon nanotube (CNT) mat electrode is demonstrated. Because of the electrical conductivity and mechanical strength of CNT mat, it can be used as an electrode as well as a catalyst support. Therefore, the preparation process for the CNT mat based sensor electrode is simpler than that of the conventional CNT dispersed sensor electrodes. GOx was covalently immobilized on the oxidized CNT mat, which is connected to a wire by using silver paste and epoxy glue. Attenuated Total Reflectance Fourier Transform-Infrared (ATR-FTIR) result shows transmittance peaks at 1637 cm(-1) and 1525 cm(-1) which are corresponding to the band I and II of amide. Cyclic voltammetric shows a pair of well-defined redox peaks with the average formal potential of -0.425 V (vs. Ag|AgCl reference electrode) in the phosphate buffered saline solution (1 x PBS, pH 7.4). Calculated electron transfer rate constant and the surface density of GOx were 1.71 s(-1) and (3.27 +/- 0.20) x 10(-13) mol/cm(2), respectively. Cyclic voltammograms of GOx-CNT mat in glucose solution show that the immobilized GOx retains its catalytic activity to glucose. The amperometric sensor response showed a linear dependence on the glucose concentration in the range of 0.2 mM to 2.18 mM with a detection sensitivity of 4.05 mu A mM(-1) cm(-2). The Michaelis-Menten constant of the immobilized GOx was calculated to be 2.18 mM.
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