期刊
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
卷 9, 期 8, 页码 4679-4685出版社
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/jnn.2009.1085
关键词
Horse Radish Peroxidase (HRP); Polyaniline; Cerium Oxide; Biosensor
类别
资金
- DST [DST/CLP 041332, DST/INT/JAP/P21/17, DST/TST/TE/2007/06, DST/SEN/Sl/03]
- CSIR [CMM-11]
- DBT [DBT/PR7667/MEP/14/1057/2006]
Hydrogen peroxide (H2O2) sensor has been fabricated by immobilizing horse radish peroxidase (HRP) onto polyaniline(PANI)-cerium oxide (CeO2) nano-composite film onto indium-tin-oxide (ITO) coated glass substrate using electrochemical technique. The HRP/PANI-CeO2/ITO bioelectrode has been characterized using scanning electron microscopy (SEM), Fourier Transform Infrared spectroscopy, UV-visible spectroscopy and electrochemical analysis, respectively. This electrode shows improved sensitivity (159.6 nA/mM) and detection limit (50 mM). Further this H2O2 sensor shows high HRP affinity to the PANI-CeO2 matrix as indicated by low value (2.9) mM of Michaelis-Menten constant (K-m). This bioelectrode exhibits maximum response current at pH 7.0 and retains 90% of its initial response current after about 8 weeks.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据