期刊
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
卷 8, 期 5, 页码 2293-2300出版社
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/jnn.2008.185
关键词
quantum dots (Qdots); liposomes; live cells
类别
资金
- NCI NIH HHS [CA096299-09] Funding Source: Medline
- NIGMS NIH HHS [GM071703] Funding Source: Medline
An increasing number of studies have demonstrated the multiple advantages of using nanocrystals, such as Quantum dots, for biological imaging. Quantum dots functionalized with biomolecules on their surfaces were shown to be able to bind to specific extracellular targets via specific recognition and to be internalized inside the cells, thereby allowing the imaging of intracellular pathways. However, the use of Quantum dots for live tracking of intracellular molecules is relatively limited because of the difficulties encountered during the induction of Quantum dots across living cell membranes. In this study we show that cationic liposomes can deliver low concentrations of non-targeted Quantum dots into the cytosol of living cells via a lipid-mediated fusion with the cell membrane. The intracellular Quantum dots exhibit aggregation that appears dependent upon their concentration, but does not visibly affect cell viability. Our results point towards the use of cationic liposomes as an effective delivery system for targeted Quantum dots within the cell cytosol, which would facilitate live cell imaging of the labeled molecules.
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