期刊
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
卷 8, 期 11, 页码 5753-5756出版社
AMER SCIENTIFIC PUBLISHERS
DOI: 10.1166/jnn.2008.206
关键词
F0F1-ATPase; DPN (Dip-Pen Nanolithography); Chromatophores; Microarray
类别
资金
- Natural Science Foundation of China [30490174, 20645003]
- CAS [Kjcx-sw-hl2, CXJJ-147]
- National Basic Research 973 [2007CB935901]
DPN (Dip-Pen nanolithography) is one kind of widely used technique to create nanoscopic patterns of many different materials. F0F1-ATPase is nano scale rotary molecular motor, and it would be an ideal motor or energy providing device in micro/nano system. In this paper, we used DPN technique to create nanoarrays of F0F1-ATPase within chromatophore on gold surface. The feature size of our F0F1-ATPase patterns was 270 nm in average, and there were no more than 20 F0F1-ATPases in each dot. The activity of patterned F0F1-ATPase was demonstrated by its ATP synthesis, which was indicated by the fluorescence change of labeled F1300. The patterned F0F1-ATPase nanoarrays can be further used as biosensor, or power providing system. And precisely patterning F0F1-ATPase with desired size, position and biological activity will accelerate its application in many basic and application research fields.
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