期刊
JOURNAL OF NANOPARTICLE RESEARCH
卷 16, 期 2, 页码 -出版社
SPRINGER
DOI: 10.1007/s11051-013-2110-7
关键词
Graphene oxide; Biogenic silver nanoparticles; Fusarium oxysporum; Antibacterial activity; Salmonella typhimurium; Microbiology
资金
- Sao Paulo State Research Foundation (FAPESP)
- National Council for Technological and Scientific Development (CNPq)
- National Institute of Science, Technology, and Innovation in Complex Functional Materials (INCT-Inomat)
- Brazilian Nanotoxicology Network (Cigenanotox)
- NanoBioss-SisNano (MCTI)
- INCT NanoBioSimes (MCT-CNPq)
- Capes-Nanobiotec network
- Funcap through the grant Pronex [PR 2-0054-00022.01.00/11]
- CNPq
- Capes
This work reports on preparation, characterization, and antibacterial activity of graphene oxide (GO) decorated with biogenic silver nanoparticles (Bio-AgNPs) produced by the fungus Fusarium oxysporum. This nanocomposite (Bio-GOAg) was prepared by an ex situ process through the physical mixture of a GO dispersion with the previously prepared Bio-AgNPs. The adsorption of the Bio-AgNPs onto the GO sheets was confirmed by transmission electron microscopy. The average size of the Bio-AgNPs anchored onto the GO surface was found to be 3.5 nm. The antibacterial activity of the Bio-GOAg nanocomposite against Gram-positive and Gram-negative microorganisms was investigated and a very promising result was found for the Gram-negative strains. In addition, the Bio-GOAg nanocomposite displayed a very strong biocidal activity against the Salmonella typhimurium strain at a concentration of 2.0 mu g/mL. The antibiofilm activity toward S. typhimurium adhered on stainless steel surfaces was also investigated. The results showed 100 % inhibition of the adhered cells after exposure to the Bio-GOAg nanocomposite for 1 h.
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