期刊
JOURNAL OF NANOPARTICLE RESEARCH
卷 15, 期 9, 页码 -出版社
SPRINGER
DOI: 10.1007/s11051-013-1930-9
关键词
Gold nanoparticles; Resonance light scattering; Chitosan; Heparin; Colorimetric detection
资金
- Natural Science Foundation of Guangdong Province [9151022501000019]
- National Natural Science Foundation of China [39670298]
In this contribution, novel chitosan-stabilized gold nanoparticles (AuNPs) were prepared by mixing chitosan with citrate-reductive AuNPs under appropriate conditions. The as-prepared chitosan-stabilized AuNPs were positively charged and highly stably dispersed in aqueous solution. They exhibited weak resonance light scattering (RLS) intensity and a wine red color. In addition, the chitosan-stabilized AuNPs were successfully utilized as novel sensitive probes for the detection of heparin for the first time. It was found that the addition of heparin induced a strong increase of RLS intensity for AuNPs and the color change from red to blue. The increase in RLS intensity and the color change of chitosan-stabilized AuNPs caused by heparin allowed the sensitive detection of heparin in the range of 0.2-60 mu M (similar to 6.7 U/mL). The detection limit for heparin is 0.8 mu M at a signal-to-noise ratio of 3. The present sensor for heparin detection possessed a low detection limit and wide linear range. Additionally, the proposed method was also applied to the detection of heparin in biological media with satisfactory results.
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