期刊
JOURNAL OF NANOPARTICLE RESEARCH
卷 14, 期 4, 页码 -出版社
SPRINGER
DOI: 10.1007/s11051-012-0736-5
关键词
Silk fibroin; Nanoparticles; Supercritical CO2; Indomethacin; Drug delivery
资金
- Hong Kong Innovation and Technology Commission
- Hong Kong Research Institute of Textile and Apparel [ITP/001/07TP, ITP/031/08TP]
- Hong Kong Research Grant Council
- Hong Kong Polytechnic University [PolyU5242/09E, G-YX1M, J-BB6Q, GU942]
- Guangdong Provincial Department of Science and Technology through the Guangdong-Hong Kong International Textile Bioengineering Joint Research Center [2011B050300023]
- Hong Kong Jockey Club Sports Medicine and Health Science Center
A novel solution-enhanced dispersion by supercritical CO2 (SEDS) was employed to prepare silk fibroin (SF) nanoparticles. The resulting SF nanoparticles exhibited a good spherical shape, a smooth surface, and a narrow particle size distribution with a mean particle diameter of about 50 nm. The results of X-ray powder diffraction, thermo gravimetry-differential scanning calorimetry, and Fourier transform infrared spectroscopy analysis of the SF nanoparticles before and after ethanol treatment indicated conformation transition of SF nanoparticles from random coil to beta-sheet form and thus water insolubility. The MTS assay also suggested that the SF nanoparticles after ethanol treatment imposed no toxicity. A non-steroidal anti-inflammatory drug, indomethacin (IDMC), was chosen as the model drug and was encapsulated in SF nanoparticles by the SEDS process. The resulting IDMC-SF nanoparticles, after ethanol treatment, possessed a theoretical average drug load of 20%, an actual drug load of 2.05%, and an encapsulation efficiency of 10.23%. In vitro IDMC release from the IDMC-SF nanoparticles after ethanol treatment showed a significantly sustained release over 2 days. These studies of SF nanoparticles indicated the suitability of the SF nanoparticles prepared by the SEDS process as a biocompatible carrier to deliver drugs and also the feasibility of using the SEDS process to reach the goal of co-precipitation of drug and SF as composite nanoparticles for controlled drug delivery.
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