期刊
JOURNAL OF NANOPARTICLE RESEARCH
卷 13, 期 4, 页码 1573-1580出版社
SPRINGER
DOI: 10.1007/s11051-010-9908-3
关键词
Gold; Nanoparticles; Polymer; Antibody; Polyallylamine; Polystyrenesulfonate; Nanomedicine; Drug delivery
资金
- Walloon Region [516071]
Nanoscale materials are used in the biomedical field for magnetic resonance imaging, protein detection and drug/gene delivery. Gold nanoparticles (AuNPs) are particularly investigated in cancer treatment and imaging. In this study, we described a simple and reliable liquid method to coat AuNPs (diameter: 21 nm) layer-by-layer with alternative cationic polyallylamine and anionic polystyrenesulfonate. The C-terminal amino acid of the antibody directed against anti-bovine serum albumin was activated by EDC/NHS, and then condensed with the amino functions of the external polyallylamine layer. An ELISA test confirmed that the antigen recognition of the bioconjugate antibody was conserved. This AuNP coating and the covalently coupling could be used as a generic process for binding other specific antibodies, particularly those overexpressed in cancer cells and angiogenesis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据