期刊
PHARMACOGNOSY MAGAZINE
卷 11, 期 44, 页码 S427-S433出版社
WOLTERS KLUWER MEDKNOW PUBLICATIONS
DOI: 10.4103/0973-1296.168982
关键词
1-diphenyl-2-pitrylhydrazyl; acute inflammation; amla; antioxidant; Emblica officinalis; high performance liquid chromatography
资金
- BT-Finishing School, Govt. of Karnataka
- Maharani Lakshmi Ammanni College for Women, Bangalore
Background: Emblica officinalis (Euphorbiaceae), popularly known as Indian gooseberry or Amla in India, is used in Ayurveda as rejuvenating herb since ancient times. Objective: This study was carried out to estimate toxicity, anti-inflammatory, and antioxidative activities of the methanolic extract of Emblica officinalis fruit (MEO) in an animal model. Materials and Methods: Antioxidative property of MEO was assessed by in vitro assays such as phosphomolybdenum assay (total antioxidant capacity), free radical scavenging assays 1,1-diphenyl-2-picrylhydrazyl and 2,2'-azino-bis and 3-ethylbenzthiazoline-6-sulphonic acid (DPPH and ABTS method) and lipid peroxidation assay (LPO). The anti-inflammatory property was evaluated by carrageenan-induced acute inflammation in rats by measuring rat paw volume at different time intervals and toxicological analysis using mice. Results and Discussion: High performance liquid chromatography studies revealed the presence of gallic acid (2,10%), mucic acid (4.90%), ellagic acid (2.10%), quercetin (28.00%), rutin (3.89%), and (beta-glucogallin (1.46%). MEO showed highest antioxidant activities by using DPPH (17.33-89.00%), ABTS (23.03-94.16%), nitric oxide scavenging activity (12.94-70.16%), LPO (56.54%), and phosphomolybdenum assay (142 +/- 6.09 mu g/ml).The LD50 was found to be approximately 1125 mg/kg (p.o). High dose of MEO showed significant reduction (72.71%) in the inflammation after 4 h of treatment, which was comparable to diclofenac (10 mg/kg) (61.57%) treated group. Significant reduction (P < 0.05) in the inflammatory cytokine (interleukin-1 beta and tumor necrosis factor-alpha) markers were also observed (57.25% and 35.41%, respectively) in serum of MEO treated animals as compared to control. Conclusion: Taken together, phenolic compounds of MEO may serve as a potential herbal drug for amelioration of acute inflammation due to their modulatory action on free radicals.
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