期刊
JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY
卷 35, 期 5-6, 页码 259-265出版社
SPRINGER
DOI: 10.1007/s10974-014-9400-2
关键词
C2C12; Skeletal myogenesis; Myotube; Luciferase reporters; Differentiation
类别
资金
- National Natural Science Foundation of China [30930054, 81170229, 31171402, 81170088, 31172044, 31071999, 30970425, 31272396, 81270156, 81270291, 81370451, 81400304, 31472060, 81470377, 81470449]
- Hunan Provincial Natural Science Foundation of China [12JJ3117, 2015JJ3087]
- Hunan Provincial Innovation Foundation For Postgraduate [CX2014B200, CX2012B213]
- Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province [2013-448-6]
- Scientific Research Fund of Hunan Provincial Education Department [14A093]
CXXC5 is a member of the CXXC-type zinc-finger domain containing protein family, which is suggested to function in gene transcription, cell adhesion and cytoskeleton organization. Previous studies have revealed that CXXC5 is expressed in skeletal muscle, but whether it regulates skeletal myogenesis is yet unknown. Here, we screened for the possible signaling pathways in which CXXC5 might participate using luciferase gene reporters. The results indicated that CXXC5 significantly increased the activities of the promoters of genes involved in skeletal muscle differentiation. We therefore studied the role of CXXC5 during skeletal myogenesis in C2C12 myoblasts. Our findings suggest that overexpression of CXXC5 in C2C12 myoblasts facilitated myocyte differentiation, while RNAi interference of CXXC5 significantly inhibited the differentiation of C2C12 myoblasts. This study suggests that CXXC5 plays a significant role in regulating skeletal myogenesis.
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