期刊
JOURNAL OF MORPHOLOGY
卷 271, 期 12, 页码 1446-1456出版社
WILEY
DOI: 10.1002/jmor.10887
关键词
barramundi; magnetic resonance microscopy; comparative morphology; perciform; central nervous system; Lates calcarifer
资金
- Queensland Government through the Queensland NMR Network
- Australian Research Council [LP0776985]
- Ridley Aquafeed
- Endeavour International Postgraduate Research Scholarship
- University of Queensland International Living Allowance
- Australian Research Council [LP0776985] Funding Source: Australian Research Council
Magnetic resonance imaging (MRI) is an established technique for morphological imaging of the central nervous system. Despite its prevalent use in a range of taxa, few studies exist on the brains of teleosts. In this study, we present a detailed analysis of a teleost brain using high-resolution MRI. Images were acquired from a Bruker 16.4 T vertical magnet with a three-dimensional flash T-2*-weighted image sequence. High contrast was obtained using Magnevist (R) and the following imaging parameters: a flip angle of 30 degrees, a repetition time of 50 ms, an echo time of 14 ms, and an image matrix of 1024 X 400 X 400. The resulting isotropic resolution of 30 mu m allowed us to thoroughly describe the architecture of the barramundi (Lates calcarifer) brain, including descriptions of nuclei, fiber tracts, and cellular layers. A good correspondence, both in contrast and morphology, was found between magnetic resonance images and Nissl-stained brain sections, allowing for an analysis of the benefits and drawbacks of MRI and conventional histology. J. Morphol. 271:1446-1456, 2010. (C) 2010 Wiley-Liss, Inc.
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