期刊
JOURNAL OF MOLECULAR STRUCTURE
卷 886, 期 1-3, 页码 77-84出版社
ELSEVIER
DOI: 10.1016/j.molstruc.2007.10.039
关键词
benzidine; hemoglobin; fluorescence spectroscopy; interaction
资金
- Educational Bureau [07KJD150235]
- Jiangsu Fundament of Qilan Project
- Scientific Foundation of Yancheng Teachers University
The interaction of benzidinne (BZ) with bovine hemoglobin (BHb) under physilolgical condition was studied by fluorescence spectrum. Fluorescence data revealed that the fluorescence quenching of BHb by BZ was the result of the formed complex of BZ-BHb, The quenching and energy transfer mechanisms were discussed, respectively. The binding constants and thermodynamic parameters at three different temperatures, the binding locality, and the binding power were obtained. The hydrophobic and hydrogen bonds interactions were the predominant intermolecular forces to stabilize the complex. The conformation of BHb was discussed by synchronous and three-dimensional fluorescence techniques. (C) 2008 Published by Elsevier B.V.
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