期刊
JOURNAL OF MOLECULAR RECOGNITION
卷 24, 期 1, 页码 23-34出版社
WILEY
DOI: 10.1002/jmr.1020
关键词
apoptosis; Bcl-2; chemical shift perturbation; FK506-binding protein; NMR spectroscopy; paramagnetic relaxation enhancement; PPIase; pseudocontact shift; X-ray crystallography
资金
- Deutsche Forschungsgemeinschaft [SFB 604]
The Bcl-2 inhibitor FKBP38 is regulated by the Ca2+-sensor calmodulin (CaM). Here we show a hitherto unknown low-affinity cation-binding site in the FKBP domain of FKBP38, which may afford an additional level of regulation based on electrostatic interactions. Fluorescence titration experiments indicate that in particular the physiologically relevant Ca2+ ion binds to this site. NMR-based chemical shift perturbation data locate this cation-interaction site within the beta 5-alpha 1 loop (Leu90-Ile96) of the FKBP domain, which contains the acidic Asp92 and Asp94 side-chains. Binding constants were subsequently determined for K+, Mg2+, Ca2+, and La3+, indicating that the net charge and the radius of the ion influences the binding interaction. X-ray diffraction data furthermore show that the conformation of the b5-a1 loop is influenced by the presence of a positively charged guanidinium group belonging to a neighboring FKBP38 molecule in the crystal lattice. The position of the cation-binding site has been further elucidated based on pseudocontact shift data obtained by NMR via titration with Tb3+. Elimination of the Ca2+-binding capacity by substitution of the respective aspartate residues in a D92N/D94N double-substituted variant reduces the Bcl-2 affinity of the FKBP38(35-153)/CaM complex to the same degree as the presence of Ca2+ in the wild-type protein. Hence, this charge-sensitive site in the FKBP domain participates in the regulation of FKBP38 function by enabling electrostatic interactions with ligand proteins and/or salt ions such as Ca2+. Copyright (C) 2010 John Wiley & Sons, Ltd.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据