期刊
JOURNAL OF MOLECULAR LIQUIDS
卷 200, 期 -, 页码 20-27出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molliq.2014.08.006
关键词
Protein-protein interaction; SAXS; Liquid-liquid phase separation; Reduced second virial coefficient
资金
- Deutsche Forschungsgemeinschaft (DFG)
- Studienstiftung des deutschen Volkes
We present an experimental study combined with a theoretical discussion of the effective interactions in protein solutions approaching a liquid-liquid phase separation (LLPS) induced by addition of multivalent metal ions. The reduced second virial coefficient, B-2/B-2(HS), is used to describe the interaction and discussed with theoretical predictions for colloidal systems. We have determined the salt and protein partitioning in the two coexisting phases, which provides the isothermal binodal of the LLPS in the (c(p), c(s)) plane. Two sets of samples, away from and at the LLPS binodal were measured by static light scattering (SLS) and small angle X-ray scattering (SAXS) to determine the second virial coefficient. In all cases, B-2/B-2(HS) is negative in the condensed regime, and increases by approaching the upper critical point in the (c(p), c(s)) plane. The results are compared with a simple colloidal model with isotropic short-ranged attraction and a thermodynamic criterion based on the reduced second virial coefficient. We discuss the application of this theoretical prediction to interpret experimental observations. (C) 2014 Elsevier B.V. All rights reserved.
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