期刊
JOURNAL OF MOLECULAR GRAPHICS & MODELLING
卷 30, 期 -, 页码 179-185出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.jmgm.2011.07.006
关键词
preQ(1) riboswitch; Molecular dynamics; Fluorescence spectroscopy; Pre-queosine; mRNA
类别
资金
- Novartis Institutes for BioMedical Research, Inc.
- NIBR Education Office
- NIBR RNAi Therapeutics
- CADD
- NITAS groups
Riboswitches are mRNA structural elements that act as intracellular sensors of small-molecule metabolites. By undergoing conformational changes capable of modulating translation or terminating transcription, riboswitches are able to play a role in regulating the concentration of essential metabolites in the cell. Computer-guided fluorescence experiments were carried out to interrogate molecular dynamics and conformational changes in the minimal riboswitch aptamer that binds 7-aminomethyl-7-deazaguanine (preQ(1)). Our combined experimental results and computational analysis suggest that the preQ, riboswitch apo form is structured but shows no evidence of a ligand-binding pocket. Simulations of the apo and bound forms indicate a large conformational change is triggered by the breaking of the Watson-Crick base pairing of nucleotides G11 and 01 upon preQ(1) removal, followed by collapse of the pocket due to interfering pi-stacking. Computational predictions of local aptamer dynamics were validated by fluorescence experiments employing 2-aminopurine substitutions. In-line probing reactions confirmed that fluorophore-labeled riboswitches retain similar higher-order structural features as the unlabeled aptamer upon ligand binding, although their affinity for the ligand is reduced by the introduction of the fluorescent reporter. (C) 2011 Elsevier Inc. All rights reserved.
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