期刊
JOURNAL OF MOLECULAR EVOLUTION
卷 73, 期 5-6, 页码 325-336出版社
SPRINGER
DOI: 10.1007/s00239-011-9479-7
关键词
NF-kappa B; Nematostella; Polymorphism; Evolution; DNA binding; Transactivation
资金
- National Science Foundation [MCB-0920461]
- ARRA [CA047763-22S3]
- Boston University [5 P42 ES07381]
- Warren-McLeod Fellowships
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [0924749] Funding Source: National Science Foundation
The NF-kappa B family of transcription factors is activated in response to many environmental and biological stresses, and plays a key role in innate immunity across a broad evolutionary expanse of animals. A simple NF-kappa B pathway is present in the sea anemone Nematostella vectensis, an important model organism in the phylum Cnidaria. Nematostella has previously been shown to have two naturally occurring NF-kappa B alleles (Nv-NF-kappa B-C and Nv-NF-kappa B-S) that encode proteins with different DNA-binding and transactivation abilities. We show here that polymorphic residues 67 (Cys vs. Ser) and 269 (Ala vs. Glu) play complementary roles in determining the DNA-binding activity of the NF-kappa B proteins encoded by these two alleles and that residue 67 is primarily responsible for the difference in their transactivation ability. Phylogenetic analysis indicates that Nv-NF-kappa B-S is the derived allele, consistent with its restricted geographic distribution. These results define polymorphic residues that are important for the DNA-binding and transactivating activities of two naturally occurring variants of Nv-NF-kappa B. The implications for the appearance of the two Nv-NF-kappa B alleles in natural populations of sea anemones are discussed.
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