期刊
JOURNAL OF MOLECULAR ENDOCRINOLOGY
卷 45, 期 1, 页码 45-57出版社
BIOSCIENTIFICA LTD
DOI: 10.1677/JME-09-0165
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资金
- Swedish Research Council
- Swedish Foundation for Strategic Research
- ALF/LUA in Gothenburg
- Lundberg Foundation
- Torsten and Ragnar Soderberg's Foundation
- Novo Nordisk Foundation
- Magnus Bergvall Foundation
- Ake Wiberg Foundation
- Tore Nilsson Foundation
- Swedish Society for Medical Research
Testosterone is a major regulator of muscle mass. Little is known whether this is due to a direct stimulation of the androgen receptor (AR) or mediated by aromatization of testosterone to estradiol (E-2), the ligand for the estrogen receptors (ERs), in peripheral tissues. In this study, we differentiated between the effects mediated by AR and ER by treating orchidectomized (orx) male mice for 5 weeks with E-2 or the non-aromatizable androgen dihydrotestosterone (DHT). Both E-2 and DHT increased muscle weight and lean mass, although the effect was less marked after E-2 treatment. Studies of underlying mechanisms were performed using gene transcript profiling (microarray and real-time PCR) in skeletal muscle, and they demonstrated that E-2 regulated 51 genes and DHT regulated 187 genes, with 13 genes (=25% of E-2-regulated genes) being regulated by both treatments. Both E-2 and DHT altered the expression of Fbxo32, a gene involved in skeletal muscle atrophy, affected the IGF1 system, and regulated genes involved in angiogenesis and the glutathione metabolic process. Only E-2 affected genes that regulate intermediary glucose and lipid metabolism, and only DHT increased the expression of genes involved in synaptic transmission and heme and polyamine biosynthesis. In summary, ER activation by E-2 treatment maintains skeletal muscle mass after orx. This effect is less marked than that of AR activation by DHT treatment, which completely prevented the effect of orx on muscle mass and was partly, but not fully, mediated via alternative pathways. Journal of Molecular Endocrinology (2010) 45, 45-57
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