期刊
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
卷 101, 期 -, 页码 101-107出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molcatb.2014.01.001
关键词
Deep eutectic solvent (DES); Ionic liquid (IL); Horseradish peroxidase (HRP); Biocatalysis; Protein structure
资金
- National Natural Science Foundation of China [21276159]
As a new type of ionic fluids that are inexpensive and environmentally friendly, deep eutectic solvents (DESs) have recently attracted a broad interest in many fields including biocatalysis. In this study, 24 DESs were prepared by mixing two cholinium salts (cholinium chloride (ChCl) and cholinium acetate (ChAc)) with four H-bond donors (HBDs) (urea, glycerol, acetamide, ethylene glycol) at three molar ratios, and their effects on the activity, stability and structure of horseradish peroxidase (HRP) have been investigated. The ChCl-based DESs have been found to be superior to the ChAc-based ones in terms of promoting the HRP activity. For the DESs composed of the same salt and same HBD, an increase in the HRP activity has been observed with a higher salt/HBD molar ratio of 1:2 < 1:1 <2:1. Structural studies with fluorescence and circular dichroism spectroscopy have agreed well with the activity data, suggesting that DESs capable of providing the enzyme with a higher alpha-helix content and a slightly more relaxed tertiary structure may facilitate the HRP activity. All the 24 DESs were able to highly stabilize the enzyme. Addition of DESs may help to improve the HRP-mediated phenolic wastewater treatment. Our experiments have also supported the idea that the extensive H-bonding network throughout the DES is sufficiently strong to prevent the DES from dissociation in aqueous solution. (C) 2014 Elsevier B.V. All rights reserved.
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