Characterization and stability studies on surfactant, detergent and oxidant stable alpha-amylase from marine haloalkaliphilic Saccharopolyspora sp. A9

A halopalkaliphilic marine Saccharopolyspora sp stain A9 with an ability to produce surfactants oxidant and detergent stable a-amylase was Isolated from marine sediments collected from west coast of India The a-amylase from strain A9 was purified to homogeneity with the aid of ammonium sulfate precipitation and gel filtration chromatography by using Sephadex G-75 insoluble corn starch and sephacryl S-100 column with a 3901-fold increase in specific activity SDS-PAGE and zymogram activity staining showed a single band equal to molecular mass of 66 kDa Enzyme was found to be stable in presence of wide range of NaCl concentration with maximum activity found at 11% (w/v) of NaCl Enzyme showed remarkable stability towards laboratory surfactants detergents and oxidants Glucose maltose and maltotnose were the main end product of starch hydrolysis indicating it is a-amylase (c) 2010 Elsevier B V All rights reserved