Isolation and characterization of novel α-amylase from marine Streptomyces sp D1

In this study, we have reported novel alpha-amylase enzyme from less extensively studied marine Streptomyces sp. D1. Enzyme production was determined by using media containing 2% sucrose. 0.35% peptone and 0.15% of malt extract. Optimum temperature for enzyme production and activity was found to be 45 degrees C and enzyme retained almost 50% of its activity at 85 degrees C. Enzyme activity was also retained in presence of commercially available detergent and oxidizing agents. The partially purified enzyme from strain D1 exhibited specific activity of 113.64 U/mg protein that corresponds to 2.8-fold Purification. SDS-PAGE and zymograin activity staining showed a single band equal to molecular mass of 66 kDa. The reported enzyme may have wide spread application for detergent and pharmaceutical industry. (C) 2008 Elsevier B.V. All rights reserved.