期刊
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
卷 58, 期 1-4, 页码 93-97出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molcatb.2008.11.011
关键词
Biodiesel fuel; Methanolysis; Partial glyceride lipase; Packed-bed reactor; Immobilized cells; Biomass support particles
资金
- Japanese Ministry of the Environment for Technical Development
The accumulation of partial glycerides such as monoglyceride (MG) and diglyceride (DG) is one of the rate-limiting steps in plant oil methanolysis catalyzed by Rhizopus oryzae producing triacylglycerol lipase. To convert partial glycerides efficiently into their corresponding methyl esters (MEs), we attempted to use a mono- and diacylglycerol lipase (mdIB) derived from Aspergillus oryzae. By considering cost efficiency, R. oryzae and recombinant mdIB-producing A. oryzae were immobilized independently within polyurethane foam biomass support particles and directly utilized as a whole-cell biocatalyst. The mdIB-producing A. oryzae effectively exhibited Substrate specificity toward MG and DG and was then used for the methanolysis of intermediate products (approximately 82% ME), which were produced using R. oryzae. In the presence of 5% water, the use of mdIB-producing A. oryzae resulted in less than 0.1% of MG and DG. whereas a considerable amount of triglyceride was present in the final reaction mixture. On the basis of these results, we developed a packed-bed reactor (PBR) system, which consists of the first column with R. oryzae and the second column containing both R. oryzae and mdIB-producing A. oryzae. Ten repeated-batch methanolysis cycles in the PBR maintained a high ME content of over 90% with MG and DC at 0.08-0.69 and 0.22-1.45%, respectively, indicating that the PBR system can be used for long-term repeated-batch methanolysis with partial glycerides at low levels. The proposed method is therefore effective for improving enzymatic biodiesel production. (C) 2008 Published by Elsevier B.V.
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