期刊
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
卷 57, 期 1-4, 页码 130-135出版社
ELSEVIER
DOI: 10.1016/j.molcatb.2008.08.005
关键词
Enzymatic conversion; Baicalin; Baicalein; beta-Glucuronidase; Biomimetic encapsulation
资金
- Natural Science Foundation of Tianjin [06YFJMJC10600]
- Cross-Century Talent Raising Program of Ministry of Education of China
- program for Changjiang Scholars and Innovative Research Team in University (PCSIRT)
- National Science Foundation of China [20576096]
- Program of Introducing Talents of Discipline to Universities [B06006]
in this study, Baicalein was produced through an enzymatic conversion catalyzed by beta-glucuronidase (GUS) encapsulated in biomimetic alginate/protamine/silica (APSi) capsules. Experimental results indicated that the thermal and pH tolerance as well as the storage and recycling stability of GUS were significantly improved after encapsulation. Under the optimum conversion conditions (37 degrees C, pH 7), a high productivity of Baicalein (73%) was obtained. No loss in enzyme activity was observed after 11-day storage and 90% of the initial activity remained after 26-day storage. No appreciable loss in activity was found during 10 repeated reaction cycles. The facile encapsulation process, the high conversion efficiency and the enhanced stability set an encouraging example for converting natural compounds into high value-added functional products. (c) 2008 Elsevier B.V. All rights reserved.
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