期刊
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
卷 51, 期 3-4, 页码 73-80出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.molcatb.2007.10.017
关键词
alcohol dehydrogenase; NADH; pyrrolidinol; reduction; chiral alcohols
Optically active N-benzyl-3-pyrrolidinois are versatile chiral building blocks. Stereoselective reduction of N-benzyl-3-pyrrolidinone is an economical and environmentally friend means of synthesizing these compounds. Devosia riboflavina KNK 10702 was discovered on screening as a source of a reducing enzyme giving the (R)-form N-benzyl-3-pyrrolidinol. An NADH-dependent alcohol dehydrogenase was purified to homogeneity through five steps from this microorganism. The relative molecular mass of the enzyme was estimated to be 58,000 on gel filtration and 28,000 on SDS-polyacrylamide gel electrophoresis. This enzyme reduced a broad range of carbonyl compounds in addition to N-substituted-3-pyrrolidinones. Some properties of the enzyme are reported herein. (C) 2007 Elsevier B.V. All rights reserved.
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