期刊
JOURNAL OF MOLECULAR BIOLOGY
卷 425, 期 14, 页码 2423-2435出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jmb.2013.03.028
关键词
gene silencing; oxidative stress; viral suppressor of RNAi; zinc binding; RYMV
资金
- French Ministry of Education and Scientific Research (Sibaghe School, University of Montpellier-II, France)
- CNRS
- IRD Institute
- University of Strasbourg UdS
- Region Alsace
- Fondation pour la Recherche Medicale
- GIS IBiSA
- NovAliX (Illkirch, Strasbourg)
Viral suppressors of RNA interference (VSRs) target host gene silencing pathways, thereby operating important roles in the viral cycle and in host cells, in which they counteract host innate immune responses. However, the molecular mechanisms of VSRs are poorly understood. We provide here biochemical and biophysical features of the dual suppressor/activator VSR P1 protein encoded by the rice yellow mottle virus. In silico analyses of P1 suggested common features with zinc finger proteins and native mass spectrometry unambiguously confirmed that recombinant P1 binds reversibly two zinc atoms, each with a different strength. Additionally, we demonstrate that the reaction of P1 with H2O2 leads to zinc release, disulfide bond formation, and protein oligomerization. A reversible protein modification by redox alterations has only been described for a limited number of zinc finger proteins and has never been reported for VSRs. Those reported here for P1 might be a general feature of Cys-rich VSRs and could be a key regulatory mechanism for the control of RNA silencing. (C) 2013 Elsevier Ltd. All rights reserved.
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