期刊
JOURNAL OF MOLECULAR BIOLOGY
卷 375, 期 3, 页码 824-836出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jmb.2007.08.067
关键词
cryo-electron microscopy; MS2; ssRNA virus; genomic RNA structure; atomic structure fitting
资金
- Engineering and Physical Sciences Research Council [GR/S51936/01, GR/S51936/02] Funding Source: researchfish
Using cryo-electron microscopy, single particle image processing and three-dimensional reconstruction with icosahedral averaging, we have determined the three-dimensional solution structure of bacteriophage MS2 capsids reassembled from recombinant protein in the presence of short oligonucleotides. We have also significantly extended the resolution of the previously reported structure of the wild-type MS2 virion. The structures of recombinant MS2 capsids reveal clear density for bound RNA beneath the coat protein binding sites on the inner surface of the T = 3 MS2 capsid, and show that a short extension of the minimal assembly initiation sequence that promotes an increase in the efficiency of assembly, interacts with the protein capsid forming a network of bound RNA. The structure of the wild-type MS2 virion at similar to 9 angstrom resolution reveals icosahedrally ordered density encompassing similar to 90% of the single-stranded RNA genome. The genome in the wild-type virion is arranged as two concentric shells of density, connected along the 5-fold symmetry axes of the particle. This novel RNA fold provides new constraints for models of viral assembly. (c) 2007 Elsevier Ltd. All rights reserved.
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