期刊
JOURNAL OF MOLECULAR BIOLOGY
卷 382, 期 3, 页码 567-572出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jmb.2008.07.044
关键词
mariner; transposon; satellite DNA; MITE; DNA recombination
资金
- The Wellcome Trust
- The European Commission [018716]
- The Spanish [CGL2006-04331]
- PAI CECJA [CVI 220]
- Ministry of Education and Science of Spain [AP2004-3841]
Although mariner transposons are widespread in animal genomes, the vast majority harbor multiple inactivating mutations and only two naturally occurring elements are known to be active. Previously, we discovered a mariner-family transposon, Mboumar, in the satellite DNA of the ant Messor bouvieri. Several copies of the transposon contain a full-length open reading frame, including Mboumar-9, which has 64% nucleotide identity to Mos1 of Drosophila mauritiana. To determine whether Mboumar is currently active, we expressed and purified the Mboumar-9 transposase and demonstrate that it is able to catalyze the movement of a transposon from one plasmid to another in a genetic in vitro hop assay. The efficiency is comparable to that of the well-characterized mariner transposon Mos1. Transposon insertions were precise and were flanked by TA duplications, a hallmark of mariner transposition. Mboumar has been proposed to have a role in the evolution and maintenance of satellite DNA in M. bouvieri and its activity provides a means to examine the involvement of the transposon in the genome dynamics of this organism. (c) 2008 Elsevier Ltd. All rights reserved.
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