期刊
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
卷 55, 期 -, 页码 117-129出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.yjmcc.2012.12.007
关键词
Fetal programming; High fat; Cardiac function; Mitochondria; Insulin; Lipotoxicity
资金
- American Heart Association [0355521Z]
- American Diabetes Association [7-0-RA-21]
Maternal overnutrition is associated with heart diseases in adult offspring, However, combined effect of maternal and postnatal fat intake on cardiac function is unknown. This study was designed to examine the impact of maternal and postnatal fat intake on metabolic, myocardial, insulin and mitochondrial responses in adult offspring. Pregnant FVB mice were fed a low fat (LF) or high fat (HF) diet during gestation and lactation. Weaning male offspring were placed on either LF or HF (calorie-restricted HF-fed mice used as weight control) for 4 months prior to assessment of metabolic indices, myocardial histology, cardiac function, insulin signaling, mitochondrial integrity and reactive oxygen species (ROS) generation. Compared with LF- and HF-fed weight-control mice, postnatal HF intake resulted in obesity, adiposity, dyslipidemia, insulin resistance, cardiac hypertrophy, interrupted cardiac contractile, intracellular Ca2+ and mitochondrial properties, all of which were significantly accentuated by prenatal fat exposure. Despite the preserved cardiac contractile function, LF offspring from HF-fed dams displayed higher body weights, increased adiposity and glucose intolerance. HF-fed mice with prenatal HF exposure displayed upregulated serine phosphorylation of IRS-1, PTP1B, the rate-limiting fatty acid synthesis enzyme stearoyl-CoA desaturase (SCD1) and hypertrophic markers (calcineurin A, GATA4, ANP, beta-MHC and skeletal alpha-actin), while suppressing AMP-dependent protein kinase, glucose uptake and PGC-1 alpha, levels. Importantly, myocardial and mitochondrial ultrastructural abnormalities were more pronounced in HF-fed offspring with prenatal fat exposure, shown as loss of mitochondrial density and membrane potential, increased ROS generation and apoptosis. Our data suggest that prenatal dietary fat exposure predisposes offspring to postnatal dietary fat-induced cardiac hypertrophy and contractile defect possibly via lipotoxicity, glucose intolerance and mitochondrial dysfunction. This article is part of a Special Issue entitled Focus on Cardiac Metabolism. (C) 2012 Elsevier Ltd. All rights reserved.
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