期刊
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
卷 52, 期 1, 页码 21-31出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.yjmcc.2011.09.020
关键词
Whole heart; Calcium; Calsequestrin; Ventricular arrhythmias; Alternans
资金
- National Institute of Health [R01HL57832, R01HL88635]
Calsequestrin (Casq2) is a low affinity Ca2+-binding protein located in sarcoplasmic reticulum (SR) of cardiac myocytes. Casq2 acts as a Ca2+ buffer regulating free Ca2+ concentration in the SR lumen and plays a significant role in the regulation of Ca2+ release from this intracellular organelle. In addition, there is experimental evidence supporting the hypothesis that Casq2 also modulates the activity of the cardiac Ca2+ release channels, ryanodine receptors (RyR2). In this study, Casq2 knockout mice (Casq2-/-) were used as a model to evaluate the effects of the Casq2 on the cytosolic and intra-SR Ca2+ dynamics, and the electrical activity in the ventricular epicardial layer of intact beating hearts. Casq2-/- mice have accelerated intra-SR Ca2+ refilling kinetics (76 +/- 22 vs. 136.5 +/- 15 ms) and a reduced refractoriness of Ca2+ release (182 +/- 32 ms Casq2+/+ and 111 +/- 22 ms Casq2-/-). In addition, mice display reduced Ca2+ alternans (67% decline in the amplitude of Ca2+ altemans at 7 Hz, 21oC) and less T-wave alternans at the electrocardiographic level. The results presented in this paper support the idea of Casq2 acting both as a buffer and a direct regulator of the Ca2+ release process. Finally, we propose that alterations in Ca2+ release refractoriness shown here could explain the relationship between Casq2 function and an increase in the risk for ventricular arrhythmias. (C) 2011 Elsevier Ltd. All rights reserved.
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