期刊
PEPTIDES
卷 71, 期 -, 页码 128-140出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.peptides.2015.07.009
关键词
Antimicrobial activity; Defensin analogs; DNA binding; Fatty acylation; Synthetic peptides
资金
- CSIR
- Department of Science and Technology, India [SR/S2/JCB-40/2010]
Human alpha-defensin 5 (HD5) exhibits broad spectrum antimicrobial activity and plays an important role in muco sal immunity of the small intestine. Although there have been several studies, the structural requirements for activity and mechanism of bacterial killing is yet to be established unequivocally. In this study, we have investigated the antimicrobial activity of HD5 and linear analogs. Cysteine deletions attenuated the antibacterial activity considerably. Candidacidal activity was affected to a lesser extent. Fatty acid conjugated linear analogs showed antimicrobial activity comparable activity to HD5. Effective surface charge neutralization of bacteria was observed for HD5 as compared to the non-fatty acylated linear analogs. Our results show that HD5 and non-fatty acylated linear analogs enter the bacterial cytoplasm without causing damage to the bacterial inner membrane. Although fatty acylated peptides exhibited antimicrobial activity comparable to HD5, their mechanism of action involved permeabilization of the Escherichia coli inner membrane. HD5 and analogs had the ability to bind plasmid DNA. HD5 had greater binding affinity to plasmid DNA as compared to the analogs. The three dimensional structure of HD5 favors greater interaction with the bacterial cell surface and also with DNA. Antibacterial activity of HD5 involves entry into bacterial cytoplasm and binding to DNA which would result in shut down of the bacterial metabolism leading to cell death. We show how a moderately active linear peptide derived from the alpha-defensin HD5 can be engineered to enhance antimicrobial activity almost comparable to the native peptide. (C) 2015 Elsevier Inc. All rights reserved.
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