期刊
PEPTIDES
卷 64, 期 -, 页码 49-54出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.peptides.2014.12.012
关键词
Angiotensin (1-7); Type 2 diabetes mellitus; Islet function; Oxidative stress; Cell apoptosis
资金
- Natural Science Foundation of Shanxi Province of China [2009011056-2]
- Programs for Science and Technology Development of Department of Public Health of Shanxi Province of China [201201074]
- Postdoctoral Science Foundation of Second Hospital of Shanxi Medical University of China [20070402]
- Programs for Science and Technology Development of Shanxi Province of China [20100311098]
The aim of this study is to determine the antidiabetic activity of Ang-(1-7), an important component of the renin-angiotensin system, in a rat model of streptozotocin (STZ)-induced type 2 diabetes mellitus (DM). A total of 36 male Wistar rats were randomly divided into 3 groups: control group fed standard laboratory diet, DM group fed high-fat diet and injected with STZ, and Ang-(1-7) group receiving injection of STZ followed by Ang-(1-7) treatment. Body weight, blood glucose levels, fasting serum Ang II and insulin levels, and homeostasis model assessment of insulin resistance (HOMA-IR) were measured. The pancreas was collected for histological examination and gene expression analysis. Notably, the Ang-(1-7) group showed a significant decrease in fasting blood glucose and serum Ang II levels and HOMA-IR values and increase in fasting serum insulin levels. Pancreatic beta cells in the control and Ang-(1-7) groups were normally distributed in the center of pancreatic islets with large clear nuclei. In contrast, pancreatic beta cells in the DM group had a marked shrinkage of the cytoplasm and condensation of nuclear chromatin. Ang(1-7) treatment significantly facilitated insulin production by beta cells in diabetic rats. The DM-associated elevation of inducible nitric oxide synthase (iNOS), caspase-3, caspase-9, caspase-8, and Bax and reduction of Bcl-2 was significantly reversed by Ang-(1-7) treatment. Taken together, Ang-(1-7) protects against STZ-induced DM through improvement of insulin resistance, insulin secretion, and pancreatic beta cell survival, which is associated with reduction of iNOS expression and alteration of the Bcl-2 family. (C) 2015 Elsevier Inc. All rights reserved.
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