期刊
JOURNAL OF MICROSCOPY
卷 244, 期 1, 页码 79-84出版社
WILEY-BLACKWELL
DOI: 10.1111/j.1365-2818.2011.03509.x
关键词
Cyanobacteria; cell suspension; high pressure freezing; pollen
类别
The high pressure freezing (HPF) followed by freeze substitution technique has advantages over chemical fixation in the context of preserving sample ultrastructure. However, when HPF is applied to cultured pollen grains, the large intercellular spaces present lead to a poor level of ultrastructure preservation. We report here that the mixing of cyanobacteria with immature barley pollen grains succeeded in greatly reducing the volume of liquid present between the large pollen grains, and so improved the loading of the sample into a nitrocellulose capillary. The use of yeast or cyanobacteria paste to surround the filled capillaries was beneficial in speeding the transfer of heat during the freezing process. This modification of the HPF method resulted in a greatly improved level of ultrastructure preservation.
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