期刊
JOURNAL OF MICROSCOPY
卷 242, 期 3, 页码 221-227出版社
WILEY-BLACKWELL
DOI: 10.1111/j.1365-2818.2010.03478.x
关键词
Vitrification; electron microscopy; tomography; image processing
类别
Cryo-electron tomography allows three-dimensional visualization of frozen-hydrated, vitrified biological material at molecular resolution. Here, we summarize the most important sample preparation methods and technical aspects relevant for cryo-electron tomography, as well as its recent biological applications from isolated macromolecular complexes to entire cells and tissues.
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