期刊
JOURNAL OF MICROSCOPY-OXFORD
卷 229, 期 1, 页码 104-114出版社
BLACKWELL PUBLISHING
DOI: 10.1111/j.1365-2818.2007.01873.x
关键词
anisotropy; FLIM; fluorescence; quadrant anode; single-photon counting; tr-FAIM; wide-field imaging
类别
A scanning-less single-photon counting system for FLIM and fluorescence anisotropy wide-field imaging is described and characterized in this paper. The two polarizations of the fluorescence are divided by a Glan prism and acquired at the same time by the Q(A) detector. Fluorescence decay profiles can be reconstructed for any desired area up to each pixel and used to calculate time-resolved fluorescence anisotropy decays.
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