期刊
JOURNAL OF MICROENCAPSULATION
卷 27, 期 2, 页码 178-186出版社
TAYLOR & FRANCIS LTD
DOI: 10.3109/02652040903059239
关键词
Swine influenza DNA vaccine; PLGA; microsphere; emulsion-evaporation method; immunology; cytotoxicity
资金
- Heilongjiang Youth Science Foundation [QC06C047]
- Department of Education of Heilongjiang Province [11513072]
- Foundation of National Key Laboratory of Veterinary Biotechnology [NKLVB-200501, NKLVB-200601]
- Innovation Foundation of Harbin [2007RFQXN098]
- Heilongjiang Postdoctoral Foundation [LBH-Q07109]
Optimal preparation conditions of DNA vaccine against swine influenza encapsulated in Poly (D, L)-lacticco-glycolic acid (PLGA) microspheres were determined. The microspheres were prepared by an emulsion-evaporation method using PLGA as the biodegradable matrix forming polymer. Using the optimal preparation conditions, PLGA microspheres containing the DNA vaccine were produced with good morphology as evident from scanning electron micrographs, high encapsulation rate and high stability. The transfection test indicated that the vaccine could be expressed as an antigen in cells and maintained good bioactivity. Moreover, these results demonstrated that the PLGA microspheres containing DNA vaccine can be used to achieve prolonged release of plasmid DNA. These results have laid a foundation for further development before ultimate industrial application.
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