期刊
JOURNAL OF MICROELECTROMECHANICAL SYSTEMS
卷 17, 期 6, 页码 1495-1500出版社
IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
DOI: 10.1109/JMEMS.2008.2006814
关键词
Antibody immobilization; biomolecule binding; evanescent wave; SU-8 waveguide
A 30-mm-long multimode waveguide, 40 mu m wide and 40 tan high, is fabricated on a silicon wafer using polymer SU-8 as the core and liquid buffer as the cladding. Antibodies are successfully immobilized on the SU-8 surface designated for binding target antigens dispersed in the buffer solution. Evanescent-wave spectroscopy is performed by exciting the fluorescently labeled antigens, bound to the waveguide surface within its evanescence field, and measuring the emission light intensity. This evanescent-wave biosensor detects specific molecular interaction. The optical output as a function of the antigen concentration can be described by Langmuir equation. Antigen concentration as low as 1.5 mu g/mL is detected; concentrations higher than 100 mu g/mL lead to sensor saturation.
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