期刊
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
卷 22, 期 2, 页码 220-225出版社
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
DOI: 10.4014/jmb.1107.07030
关键词
Mixed culture; pH control strategy; PVA-degrading enzyme; PVA dehydrogenase; oxidized PVA hydrolase
资金
- Program from the Major State Basic Research Development Program of China (973 Program) [2007CB 714036]
- National Science Fund for Distinguished Young Scholars [20625619]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
In batch culture for Poly(vinyl alcohol) (PVA)-degrading enzyme (PVAase) production by a mixed culture, higher pH (pH 7.5) was favorable for PVAase production at the prophase of cultivation, but lower pH (pH 7.0) was favorable at the anaphase. This situation was caused by the fact that the optimum pH for different key enzymes [PVA dehydrogenase (PVADH) and oxidized PVA hydrolase (OPH)] production is various. The activity and average specific production rate of PVADH reached the highest values at constant pH 7.5, whereas those of OPH appeared at pH 7.0. A two-stage pH control strategy was therefore developed and compared for its potential in improving PVAase production. By using this strategy, the maximal PVAase activity reached 2.05 U/ml, which increased by 15.2% and 24.2% over the fermentation at constant pH 7.5 and 7.0.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据