期刊
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
卷 21, 期 2, 页码 183-186出版社
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
DOI: 10.4014/jmb.1001.01017
关键词
Exopolymeric substances (EPS); pozzolana; biofilm stability; enzymatic and chemical treatments; Thiomonas arsenivorans
资金
- COBIAS Project (ANR-PRECODD) [0794C0049]
- BRGM Research Division [PDR09DR04/09EC29]
Biochemical methods were selected to evaluate the role of exopolymeric substances in the stability of biofilms used in bioremediation processes. Biofilms of Thiomonas arsenivorans formed on pozzolana were thus treated with pronase (protein target), lectins (Con A or PNA), calcofluor or periodic acid (polysaccharides target), DNase (DNA target), and lipase (triglycerides target). Neither protease nor DNase treatments had any effect on bacterial adhesion. Lectins and calcofluor treatments mainly affected young biofilms. Lipase treatment had a noticeable effect on biofilm stability whatever the biofilm age. Results suggest that it would be an increased resistance of mature biofilms that protects them from external attacks.
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