Cloning, Expression, Purification, and Properties of an Endoglucanase Gene (Glycosyl Hydrolase Family 12) from Aspergillus niger VTCC-F021 in Pichia pastoris

A gene coding for an endoglucanase (EgIA), of the glycosyl hydrolase family 12 and derived from Aspergillus niger VTCC-F021, was cloned and sequenced. The cDNA sequence, 717 bp, and its putative endoglucanase, a 238 aa protein with a predicted molecular mass of 26 kDa and a pI of 4.35, exhibited 98.3-98.7% and 98.3-98.6% identities, respectively, with cDNA sequences and their corresponding endoglucanases from Aspergillus niger strains from the GenBank. The cDNA was overexpressed in Pichia pastoris GS115 under the control of an AOX1 promoter with a level of 1.59 U/ml culture supernatant, after 72 h of growth in a YP medium induced with 1% (v/v) of methanol. The molecular mass of the purified EgIA, determined by SOS PAGE, was 33 kDa, with a specific activity of 100.16 and 19.91 U/mg toward 1% (w/v) of beta-glucan and CMC, respectively. Optimal enzymatic activity was noted at a temperature of 55 degrees C and a pH of 5. The recombinant EgIA (rEgIA) was stable over a temperature range of 30-37 degrees C and at pH range of 3.5-4.5. Metal ions, detergents, and solvents tested indicated a slightly inhibitory effect on rEgIA activity. Kinetic constants (K-m, V-max, k(cat), and k(cat)/K-m) determined for rEgIA with beta-glucan as a substrate were 4.04 mg/ml, 102.04 U/mg, 2,040.82 min(-1), and 505.05, whereas they were 10.17 mg/ml, 28.99 U/mg, 571.71 min(-1), and 57.01 with CMC as a substrate, respectively. The results thus indicate that the rEgIA obtained in this study is highly specific toward beta-glucan. The biochemical properties of rEgIA make it highly valuable for downstream biotechnological applications, including potential use as a feed enzyme.