期刊
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
卷 21, 期 7, 页码 753-756出版社
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
DOI: 10.4014/jmb.1102.01055
关键词
Methylocystis sp.; methane oxidation; aromatic compound; sulfur compounds
资金
- Ministry of Education, Science and Technology (MEST) [R0A-2008-000-20044-0]
- Advanced Environmental Biotechnology Research Center at Pohang University of Science and Technology of the NRF, MEST [2009-0079504]
- National Research Foundation of Korea [R11-2003-006-06001-0] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
A methane-oxidizing bacterium was isolated from the enriched culture of a landfill cover soil. The closest relative of the isolate, designated M6, is Methylocystis sp. Based on a kinetic analysis, the maximum specific methane oxidation rate and saturation constant were 4.93 mmol.g-dry cell weight(-1).h(-1) and 23 mu M, respectively. This was the first time a kinetic analysis was performed using pure methanotrophic culture. The methane oxidation by M6 was investigated in the presence of aromatic (m- and p-xylene and ethylbenzene) or sulfur (hydrogen sulfide, dimethyl sulfide, methanthiol) compounds. The methane oxidation was inhibited by the presence of aromatic or sulfur compounds.
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