Implications of Fullerene-60 upon in-vitro LDPE Biodegradation

Fullerene-60 nanoparticles were used for studying their effect on the low-density polyethylene (LDPE) biodegradation efficiency of two potential polymer-degrading consortia comprising three bacterial strains each. At a concentration of 0.01% (w/v) in minimal broth lacking dextrose, fullerene did not have any negative influence upon the consortia growth. However, fullerene was found to be detrimental for bacterial growth at higher concentrations (viz., 0.25%, 0.5%, and 1%). Although addition of 0.01% fullerene into the biodegradation assays containing 5 mg/ml LDPE subsided growth curves significantly, subsequent analysis of the degraded products revealed an enhanced biodegradation. Fourier transform infrared spectroscopy (FT-IR) revealed breakage and formation of chemical bonds along with the introduction of nu C-O frequencies into the hydrocarbon backbone of LDPE. Moreover, simultaneous thermogravimetric differential thermogravimetry differential thermal analysis (TG-DTG-DTA) revealed a higher number of decomposition steps along with a 1,000-fold decrease in the heat of reactions (Delta H) in fullerene-assisted biodegraded LDPE, suggesting the probable formation of multiple macromolecular by-products. This is the first report whereby fullerene-60, which is otherwise considered toxic, has helped to accelerate the polymer biodegradation process of bacterial consortia.