期刊
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
卷 19, 期 2, 页码 187-193出版社
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
DOI: 10.4014/jmb.0804.292
关键词
EstY; freshwater metagenome; lipolytic enzyme family; phylogenetic tree
资金
- National High-Tech Research and Development Program of China [2007AA09Z421]
- One Hundred Talent Project of the Chinese Academy of Sciences
A metagenomic library of surface-water microbes from the Yangtze River in China was constructed, and a novel esterase, designated as EstY, was isolated and characterized. EstY had 423 amino acids with an estimated molecular mass of 44 kDa and pI of 7.28. It hydrolyzed various p-nitrophenyl esters (acetate, butyrate, caprate, caprylate, laurate, myristate, and palmitate) and its best substrate was p-nitrophenyl caprate (C8). The optimum pH for EstY activity was 9.0 and the optimum temperature was 50 degrees C. Metal ions, such as Mn2+, Co2+, Hg2+, Zn2+, and Fe3+, strongly inhibited the activity of EstY, whereas Mg2+ was required for maximal activity. Activity remained in the presence of 10% alcohol, acetone, isopropanol, and dimethyl sulfoxide, respectively. An analysis of the amino acid sequence deduced from estY revealed that it had 7 closely related lipolytic enzymes. Moreover, a sequence analysis showed that EstY, like its 7 relatives, did not belong to any known lipolytic enzyme family.
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